BL21T7LG competent cells:
BL21T7LG is a derivative of the BL21 strain. It has a chromosomally integrated plasmid pT7LG that expresses the T7 RNA polymerase gene from Plac/O2 (lacUV5 promoter and 2x lacO). The pT7LG plasmid also contains a lacIq gene and gentamycin resistance gene. Thus, expression of the T7 RNA polymerase gene is tightly regulated by IPTG. The chemically competent BL21T7LG cells are suitable for protein expression, and as it tightly controls the expression of the T7 RNA polymerase, it is especially suitable for expression of toxic proteins.
Genotype: E. coli B F– ompT gal dcm lon hsdSB(rB– mB–) [malB+]K-12(λS) pT7LG (GenR)
Transformation efficiency: ~5 x 104 cfu/µg pUC19 DNA
Shipped in: dry ice