BL21T7AG competent cells:
BL21T7AG is a derivative of the BL21 strain. It has a chromosomally integrated plasmid pT7AG that expresses the T7 RNA polymerase gene from the PBad promoter (an arabinose-inducible promoter). The pT7AG plasmid carries a gentamycin resistance gene. Thus, expression of the T7 RNA polymerase gene is tightly regulated by arabinose. The chemically competent BL21T7AG cells are suitable for protein expression, and as it tightly controls the expression of the T7 RNA polymerase, it is especially suitable for expression of toxic proteins.
Genotype: E. coli B F– ompT gal dcm lon hsdSB(rB– mB–) [malB+]K-12(λS) pT7AG (GenR)
Transformation efficiency: ~5 x 104 cfu/µg pUC19 DNA
Shipped in: dry ice